‘Biochemiresistor’ sensor is fast, super-sensitive

June 1, 2012

A resistance-based biosensor uses gold-coated magnetic nanoparticles (Au@MNPs) functionalized with the antibiotic enrofloxin (red), which bind to anti-enrofloxin as analyte (blue). The Au@MNPs can be magnetically assembled between electrodes; the measured resistance R is a function of analyte concentration. (Credit: Dr. Leo M. H. Lai et al./Angewandte Chemie)

A new class of biosensor that can detect exceptionally small traces of contaminants in liquids in just 40 minutes has been developed by a University of New South Wales (UNSW)-led team of researchers.

Known as a biochemiresistor, it meets a long-standing challenge to create a sensor that is both super-sensitive to the presence of chemical compounds and responds quickly.

It has countless potential uses for detecting drugs, toxins and pesticides for biomedical or environmental analysis.

The researchers tested the new sensor by detecting tiny traces in milk of the veterinary antibiotic enrofloxacin, an antibiotic used in the agricultural industry that can be transferred to the food chain, according to Scientia Professor Justin Gooding, of the UNSW School of Chemistry and the Australian Centre for Nanomedicine.

“Our biochemiresistor was able to detect enrofloxacin in neat milk in 40 minutes, at level as low as one nanogram in a liter of milk. To put that number in perspective, a nanogram is a billionth of a gram and is the mass of a single cell,” he said.

A biosensor is a portable analytical device that uses biological molecules to detect selectively just one compound within a mix of many others. Small biosensors are already in daily use testing the safety of drinking water, for checking diabetic blood-sugar levels and for pregnancy tests

The biochemiresistor uses gold-coated magnetic nanoparticles modified with antibodies that are selective for the chemical constituent (analyte) of interest. The nanoparticles are dispersed into the sample for analysis and if the analyte is present, some of the antibodies detach from the nanoparticles.

Using a magnet, the nanoparticles are then assembled into a film between two electrodes and the electrical resistance is measured. The more analyte is present, the more antibodies leave the nanoparticles and the lower the resistance in the nanoparticle film.


The nanoparticles are assembled over interdigitated electrodes (credit: Dr. Leo M. H. Lai et al./Angewandte Chemie)

“This new type of biosensor is rapid in response because the magnetic nanoparticle biosensors go and get the analyte rather than the usual approach of waiting for the analyte to find the sensing surface,” says Gooding.

“The biochemiresistor is also more sensitive than the usual biosensor because, as the nanoparticles are dispersed throughout the sample, the entire sample is analysed, not just a small portion of the solution.”

The team included other researchers from the Australian Centre for NanoMedicine and the former ARC Centre of Excellence for Functional Nanomaterials at UNSW.

Dr. Leo M. H. Lai et al., The Biochemiresistor: An Ultrasensitive Biosensor for Small Organic Molecules, Angewandte Chemie, 2012, DOI: 10.1002/anie.201202350