Merging nanoelectronics into 3D engineered human tissues

Researchers grow cyborg tissues with embedded nanoelectronics
August 28, 2012
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3D reconstruction confocal microscopy image of a 3D macroporous nanoelectronic scaffold; a nanoelectronic device exists (too small to see in image) in each circular region (credit: Harvard University)

Harvard scientists have created a type of “cyborg” tissue for the first time by embedding a three-dimensional network of functional, biocompatible, nanoscale wires into engineered human tissues.

The research team led by Charles M. Lieber, the Mark Hyman Jr. Professor of Chemistry at Harvard, and Daniel Kohane, a Harvard Medical School professor in the Department of Anesthesia at Children’s Hospital Boston, developed a system for creating nanoscale “scaffolds” that can be seeded with cells that grow into tissue.

“The current methods we have for monitoring or interacting with living systems are limited,” said Lieber. “We can use electrodes to measure activity in cells or tissue, but that damages them. With this technology, for the first time, we can work at the same scale as the unit of biological system without interrupting it. Ultimately, this is about merging tissue with electronics in a way that it becomes difficult to determine where the tissue ends and the electronics begin.”

Contributing to the work were Robert Langer, from the Koch Institute at the Massachusetts Institute of Technology, and Zhigang Suo, the Allen E. and Marilyn M. Puckett Professor of Mechanics and Materials at Harvard’s School of Engineering and Applied Sciences.

3D reconstructed confocal microscopy image of synthetic 3D neural tissue with neurons (red) and nanoelectronic circuitry (green/blue)  (credit: Tian, et al./Harvard University)

The research addresses a concern that has long been associated with work on bioengineered tissue: how to create systems capable of sensing chemical or electrical changes in the tissue after it has been grown and implanted.

The system might also represent a solution to researchers’ struggles in developing methods to directly stimulate engineered tissues and measure cellular reactions.

“In the body, the autonomic nervous system keeps track of pH, chemistry, oxygen, and other factors, and triggers responses as needed,” Kohane said. “We need to be able to mimic the kind of intrinsic feedback loops the body has evolved in order to maintain fine control at the cellular and tissue level.”

Building 3D networks of nanoscale sensors

Using the autonomic nervous system as inspiration, Bozhi Tian, a former doctoral student under Lieber and a former postdoctoral fellow in the Kohane and Langer labs, joined with Harvard graduate student Jia Liu in Lieber’s Harvard lab to build meshlike networks of nanoscale silicon wires.

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Scanning electron microscopy image of one circular region of the nanoelectronic scaffold, with nanowire transistor at the center. (credit: Harvard University)

The process of building the networks, Lieber said, is similar to that used to etch microchips.

Beginning with a two-dimensional substrate, researchers laid out a mesh of organic polymer around nanoscale wires, which serve as the critical sensing elements. Nanoscale electrodes, which connect the nanowire elements, were then built within the mesh to enable nanowire transistors to measure the activity in cells without damaging them.

Once completed, the substrate was dissolved, leaving researchers with a netlike sponge, or a mesh, that can be folded or rolled into a host of three-dimensional shapes.

Once complete, the networks were porous enough to allow the team to seed them with cells and encourage those cells to grow in 3-D cultures.

“Previous efforts to create bioengineered sensing networks have focused on two-dimensional layouts, where culture cells grow on top of electronic components, or on conformal layouts, where probes are placed on tissue surfaces,” said Tian.

“It is desirable to have an accurate picture of cellular behavior within the 3-D structure of a tissue, and it is also important to have nanoscale probes to avoid disruption of either cellular or tissue architecture.”

Schematic of the conceptual basis for the 3D networks of nanoscale sensors  (credit: Tian, et al./Harvard University)

Using heart and nerve cells, the team successfully engineered tissues containing embedded nanoscale networks without affecting the cells’ viability or activity. Using the embedded devices, the researchers were then able to detect electrical signals generated by cells deep within the tissue, and to measure changes in those signals in response to cardio- or neuro-stimulating drugs.

They were also able to construct bioengineered blood vessels, and used the embedded technology to measure pH changes — as would be seen in response to inflammation, ischemia, and other biochemical or cellular environments — both inside and outside the vessels.

Though a number of potential applications exist for the technology, the most near-term use, Lieber said, may come from the pharmaceutical industry, where researchers could use it to more precisely study how newly developed drugs act in three-dimensional tissues, rather than thin layers of cultured cells. The system might also one day be used to monitor changes inside the body and react accordingly, whether through electrical stimulation or the release of a drug.

The study was supported by the National Institutes of Health, the McKnight Foundation, and Children’s Hospital Boston.